Method for producing an enzyme cocktail using the solid residues from a process for biochemically converting of lignocellulosic materials

摘要

The present invention relates to a method for producing an enzyme cocktail in a submerged culture with a cellulolytic microorganism, including two phases: a phase a) of growing said microorganism in the presence of at least one carbon growth substrate in a closed reactor, the concentration of said carbon growth substrate in said growing phase being between 10 and 90 g/L; and a phase b) for producing the enzyme cocktail in which at least one inducing carbon substrate is fed, said inducing carbon substrate being at least one solid residue from the enzymatic hydrolysis of lignocellulosic materials having undergone a pretreatment step, the concentration of the carbon production substrate in said production phase being between 150 and 400 g/L.