| 摘要 |
<p>The invention provides methods for gene synthesis and error correction on a droplet actuator. In one embodiment, the invention provides a method for performing polymerase-mediated gene synthesis on a droplet actuator. The polymerase-mediated gene synthesis method uses oligonucleotide hybridization and PCR cycling to generate a pool of synthesized DNA strands, which may be subsequently enriched for the correct gene sequence.</p> |
