METHODS FOR TRANSFORMING TARWI AND FOR PRODUCING MOLECULAR FARMING PRODUCTS IN TRANSGENIC TARWI SEED

摘要

The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.

主权项

1. A method of producing transformed tarwi comprising a human adenosine deaminase (hADA) or a functional variant thereof, the method comprising:
slicing at least one tarwi seed embryo to produce at least one explant; infecting the explant by incubating the explant in a co-cultivation medium comprising agrobacterium, wherein the agrobacterium comprises a polynucleotide sequence encoding the human adenosine deaminase (hADA) or functional variant thereof; thereby generating a transformed explant; elongating at least one transformed shoot from the transformed explant to form an elongated shoot; and regenerating a transformed tarwi plant from the elongated shoot.