IMMUNOGLOBULIN-BOUND EXTRACELLULAR VESICLES AND USES THEREOF

摘要

Provided are methods of isolating IgG-bound (e.g., IgG-bound or protein G-recognized IgG-bound) extracellular vesicles from a sample containing a biological fluid from a subject, where the IgG (e.g., IgG2 or protein G-recognized IgG) is bound to an antigen present on the surface of the extracellular vesicle and the IgG (e.g., IgG2 or protein G-recognized IgG) is an endogenous antibody. Also provided are methods of diagnosing a cancer in a subject that include detecting the presence of one or more tumor antigens in an isolated IgG-bound (e.g., IgG2-bound or protein G-recognized IgG-bound) extracellular vesicle, and methods of treating a subject that include administering one or more cancer therapeutics to a subject having an IgG-bound (e.g., IgG2-bound or protein G-recognized IgG-bound) extracellular vesicle that contains one or more tumor antigens.

主权项

1. A method of isolating a protein G-recognized IgG-bound extracellular vesicle, wherein the protein G-recognized IgG is bound to an antigen present on the surface of the extracellular vesicle and the protein G-recognized IgG is an endogenous antibody, the method comprising:
(a) obtaining a sample comprising a biological fluid from the subject, (b) contacting the sample with an agent that specifically binds to a heavy chain constant domain of one or more protein G-recognized IgG, optionally wherein the protein G-recognized IgG is IgG2, (c) separating the agent bound with protein G-recognized IgG-bound extracellular vesicles from unbound material present in the sample, and (d) dissociating the protein G-recognized IgG-bound extracellular vesicles from the agent, thereby isolating protein G-recognized IgG-bound extracellular vesicles.