| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for highly resolved optical scanning of a sample, which uses a laser scanning fluorescence microscope.SOLUTION: In the method, a sample 10 comprises a substance which can be shifted to different energy states (a first state Z1 and a second state Z2), and the sample is illuminated with light at a wavelength of an excitation spectrum of the substance for locally producing the first state Z1 of the substance and is illuminated with light at a suitable deexcitation wavelength in a focus edge region of the excitation for producing the second state Z2 of the substance, and emission light emanating from the sample and resulting from a decay of the remaining first state Z1 is detected by a detection device 89. The sample comprises at least one further substance which can be converted to a first state Z1' and a second state Z2', and respective substances differ from each other in at least one characteristic and at least partially match each other in at least one other characteristic, and the steps of the excitation and/or the deexcitation and/or the detection are carried out separately from one another in terms of time for the individual substances during scanning of the sample.</p> |
