METHODS AND COMPOSITIONS FOR TREATMENT OF TRAUMATIC BRAIN INJURY AND FOR MODULATION OF MIGRATION OF NEUROGENIC CELLS

摘要

Disclosed herein are methods for the treatment of traumatic brain injury by transplantation of cells descended from marrow adherent stem cells that express an exogenous Notch intracellular domain. The transplanted cells form a pathway along which endogenous neurogenic cells proliferate and migrate from the subventricular zone to the site of injury.

主权项

1. A method for treating traumatic brain injury in a subject, the method comprising administering, to the brain of the subject, a therapeutically effective amount of SB623 cells, wherein the SB623 cells are obtained by
(a) providing a culture of marrow adherent stem cells (MSCs), (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein, (c) selecting cells that comprise the polynucleotide of step (b), and (d) further culturing the selected cells of step (c) in the absence of selection.