| 摘要 |
<p>Provided are methods of isolating IgG-bound (e.g., IgG-bound or protein G-recognized IgG-bound) extracellular vesicles from a sample containing a biological fluid from a subject, where the IgG (e.g., IgG2 or protein G-recognized IgG) is bound to an antigen present on the surface of the extracellular vesicle and the IgG (e.g., IgG2 or protein G-recognized IgG) is an endogenous antibody. Also provided are methods of diagnosing a cancer in a subject that include detecting the presence of one or more tumor antigens in an isolated IgG-bound (e.g., IgG2-bound or protein G-recognized IgG-bound) extracellular vesicle, and methods of treating a subject that include administering one or more cancer therapeutics to a subject having an IgG-bound (e.g., IgG2-bound or protein G-recognized IgG-bound) extracellular vesicle that contains one or more tumor antigens.</p> |
