OPTICAL ANALYSING DEVICE AND METHOD USING INDIVIDUAL LIGHT-EMITTING PARTICLE DETECTION

摘要

There is provided an optical analysis technique of detecting light of a light-emitting particle in a sample solution in the scanning molecule counting method using the light measurement with a confocal microscope or a multiphoton microscope, for suppressing the scattering in detected results of signals of light of light-emitting particles smaller and achieving the improvement of accuracy. The inventive optical analysis technique is characterized by moving the position of a light detection region of the optical system of the microscope along a predetermined route at least two circulation times by changing the optical path of the optical system; detecting light from the light detection region and generating time series light intensity data during the moving of the position of the light detection region along the predetermined route of multiple times and detecting individually a signal indicating light from each light-emitting particle existing in the predetermined route using the time series light intensity data obtained in the circulating movement of the light detection region along the predetermined route of multiple times.