| 摘要 |
The invention relates to methods for coating a substrate with at least one monolayer of self-assembling proteins, using stabilized aqueous solutions with self-assembling proteins, and also to substrates obtainable as a result. Methods for stabilizing solutions with self-assembling proteins, and the stabilized solutions obtainable therefrom, are likewise provided by the invention. According to the coating method, at least one monolayer of a self-assembling protein is produced on a substrate by first providing a stabilized aqueous solution which comprises at least one self-assembling protein. To provide the coating solution, protein units aggregated from an aqueous solution of self-assembling proteins are separated off, and addition of a solution of ionic surfactants and/or of a salt-containing and/or alkaline and/or acidic solution to the protein-containing coating solution generates monomers or oligomers of the self-assembling proteins, and stabilizes them, the amount of ionic surfactant added being such that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. A substrate surface is then brought into contact with the stabilized, protein-containing solution, thus producing a protein-containing coating on the substrate. The supernatant solution is removed from the coated substrate and/or the coated substrate is dried. |
