| 摘要 |
<p>A poliomyelitis vaccine product is prepared by absorbing the virus antigen from a killed aqueous vaccine containing at least one strain of human poliomyelitis antigen on to aluminium phosphate gel, separating the latter with the absorbed antigen and resuspending in water or a physiological salt solution. The suspension is substantially free from proteins. In preparing the starting vaccine, trypsinized macerated monkey kidney tissue is inoculated with the virus the mixture is incubated, and the living virus in the harvested fluid is inactivated with formaldehyde, ultra-violet radiation, &c. Absorption of the antigen is effected below 40 DEG C., preferably at 0-10 DEG C. The resuspension may contain 0.2-8 mg./ml. aluminium phosphate, and benzethorium chloride in concentration 1 to 10,000-50,000 as a preservative. Other antigens, e.g. tetanus, diphtheria, pertussis may also be absorbed in the aluminium phosphate. Tabulated results show that the original vaccine is less potent than the original vaccine plus aluminium phosphate and still less potent than the separated and resuspended vaccine product.</p> |
