| 摘要 |
<p>An apparatus for cutting a biospecimen workpiece W into a section while maintaining the living state of cells comprises a board 4 on which the workpiece is placed, a device for freezing and fixing the workpiece on the board, and a blade 1 for cutting the frozen workpiece fixed on the board into a section by means of rotary movement in a predetermined rotational direction r. The device for freezing may be a liquid nitrogen dripping device 8. A profile of a cutting edge of the blade is a curve showing monotonic increase in distances of a rotational axis to points starting at a nearest point that is the shortest from the axis and ending at a farthest point that is the longest from the axis. The farthest point is behind the nearest point in the rotational direction and the curve is convex opposite to the rotational axis with respect to a straight line connecting the nearest point and the farthest point (Q, fig3). The apparatus may be used in a method for cell observation that uses a phase-contrast microscope to observe the cells while the section is immersed in a tissue culture growth medium.</p> |
