Colorimetric absorbance measurement method, apparatus and system

摘要

Various embodiments disclose a colorimetric absorbance measurement method and a system for performing the same. The method comprises driving a reaction tray carrying a plurality of reaction cuvettes to rotate at a speed; transferring or rotating filters on a filter wheel to a light path of a light beam, starting from a filter of a first wavelength; and sampling photoelectric data when the light beam has passed through each reaction cuvette in some embodiments. The method ensures consistency in the measurement and synchronization between the calibration and the sample test and reliability of the measurements. Various embodiments simplify data processing and reduce the complexity of the system.

主权项

1. A colorimetric absorbance measurement method, comprising:
emitting a light beam via a light source; driving a reaction tray carrying a plurality of reaction cuvettes to rotate at a substantially uniform speed through the light beam; transferring one or more filters which are arranged as or on a filter wheel sequentially to or in a light path of the light beam, starting from a filter of a first wavelength; halting the filter wheel at one or more time intervals; sampling, via a photoelectric detection unit, photoelectric data at the one or more time intervals when the filter wheel is halted and the light beam has passed through at least one of the plurality of reaction cuvettes; and controlling, via a controller, the timing of halting the filter wheel and the timing of sampling photoelectric data; calculating, via a processor, a colorimetric absorbance using the sampled photoelectric data.