发明名称 |
Reprogramming immortalized B-cells to induced pluripotent stem cells |
摘要 |
Methods and composition for providing induced pluripotent stem (iPS) cells are provided. For example, in certain aspects methods including reprogramming B lymphocytes transformed by episomal vectors such as Epstein-Barr virus-based vectors are described. Furthermore, the invention provides induced pluripotent stem cells essentially free of exogenous elements and having B cell immunoglobin variable region rearrangement. |
申请公布号 |
US8765470(B2) |
申请公布日期 |
2014.07.01 |
申请号 |
US201113197529 |
申请日期 |
2011.08.03 |
申请人 |
Cellular Dynamics International, Inc. |
发明人 |
Thomson James;Rajesh Deepika;Dickerson Sarah Jane;Mack Amanda;Miller Michael |
分类号 |
C12N15/00;C12N15/63;C12N15/85 |
主分类号 |
C12N15/00 |
代理机构 |
Parker Highlander PLLC |
代理人 |
Parker Highlander PLLC |
主权项 |
1. A method for producing human induced pluripotent stem cells (iPS cells) from immortalized B cells, wherein the method comprises:
a) obtaining immortalized human B cells that have been immortalized by introducing one or more immortalizing Epstein-Barr virus (EBV) elements comprising EBNA into B cells; b) reprogramming the immortalized B cells by introducing an exogenous reprogramming episomal vector or vectors encoding exogenous reprogramming factors comprising at least Oct-4 and Sox-2; and c) culturing said cells under defined conditions in the absence of a feeder layer to effect expression of the exogenous reprogramming factors therein for sufficient time to produce iPS cells; and d) removing the EBV and episomal vector elements from the iPS cells to provide human iPS cells that are essentially free of exogenous EBV and episomal vector elements. |
地址 |
Madison WI US |