| 摘要 |
Disclosed is a novel HIV gene comprising a set of open reading frames encoded with the template as the plus strand of the proviral DNA, and located in the region of HIV-1 long terminal repeat. The genes encode a set of antisense proteins, (HAPs) as well as smaller proteins, related to, and containing structural motif resembling that of chemokine proteins. Depending upon the ribosomal frameshift, a plurality of proteins may be translated from the antisense RNA. The smaller proteins have similarity with chemokine SDF-1 and may play a role as a cofactor with gp120 in the binding to and entry of HIV to a target cell. |
| 主权项 |
1. A recombinant expression vector containing a heterologous antisense gene of the human immunodeficiency virus (HIV) which encodes and produces an antisense RNA polynucleotide and/or an HIV antisense proteins (HAP) or polypeptides, wherein the antisense RNA polynucleotide consists of SEQ ID NO:27, SEQ ID NO:32, or SEQ ID NO:34, and wherein the HAP protein or polypeptide consists of an amino acid sequence that is at least 80% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO:35, and SEQ ID NO:36. |
