| 摘要 |
Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1). |
| 主权项 |
1. An isolated polypeptide having xylanase activity and comprising an amino acid sequence, said amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 1, and which polypeptide has
i) one or two amino acid substitutions in a position selected from the group consisting of: 12 and 13; and ii) one or more further amino acid substitutions in a position selected from the group consisting of: 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175, and 179, wherein said positions are determined as the position corresponding to the position of B. subtilis xylanase sequence shown as SEQ ID NO: 1 by alignment; wherein the amino acid modification in position 12 is an amino acid substitution to any one different amino acid residue selected from the group consisting of: isoleucine, alanine, leucine, asparagine, lysine, aspartic acid, methionine, cysteine, glutamic acid, threonine, glutamine, tryptophan, valine, proline, serine, tyrosine, arginine, and histidine; and wherein the amino acid substitution in position 13 is an amino acid substitution to any one different amino acid residue selected from the group consisting of: alanine, leucine, asparagine, lysine, aspartic acid, methionine, cysteine, glutamic acid, threonine, glutamine, tryptophan, valine, proline, serine, tyrosine, arginine, and histidine. |
