Polynucleotide encoding a maltotriosyl transferase

摘要

The object is to provide a novel glycosyltransferase and the use thereof, the glycosyltransferase catalyzes transglucosylation of maltotriose units under conditions which can be employed for the processing of foods or the like. Provided is a maltotriosyl transferase which acts on polysaccharides and oligosaccharides having α-1,4 glucoside bonds, and has activity for transferring maltotriose units to saccharides, the maltotriosyl transferase acting on maltotetraose as substrate to give a ratio between the maltoheptaose production rate and maltotriose production rate of 9:1 to 10:0 at any substrate concentration ranging from 0.67 to 70% (w/v).

主权项

1. A recombinant expression vector comprising an isolated polynucleotide encoding a maltotriosyl transferase, wherein the polynucleotide comprises a DNA selected from the group consisting of the following (a) or (b):
(a) a DNA encoding the amino acid sequence set forth in SEQ ID NO: 7 or 8; and (b) a DNA comprising the nucleotide sequence set forth in SEQ ID NO: 6.