Method for in vivo residue-specific DOPA incorporation into mussel adhesive proteins

摘要

The present invention relates to recombinant mussel adhesive protein wherein a DOPA residue is in vivo incorporated instead of a tyrosine residue, and a method for producing the same. More specifically, the present invention relates to recombinant mussel adhesive protein wherein a DOPA residue is incorporated instead of a tyrosine residue, and a method for producing the same, and a transformant for producing the recombinant mussel adhesive protein.

主权项

1. A method for producing mussel adhesive protein wherein a tyrosine residue is substituted by DOPA or DOPA o-quinone, comprising the steps of:
(1) introducing a vector that comprises nucleic acid encoding mussel adhesive protein in tyrosine auxotroph to prepare a transformant; (2) culturing the prepared transformant in a tyrosine-free medium to a stationary phase; and (3) when the cells reach a stationary phase, adding DOPA (3,4-dihydroxyphenylalanine) or DOPA o-quinone to the medium, and additionally culturing.