<i>Brassica ogura </i>restorer lines with shortened <i>Raphanus </i>fragment (SRF)

摘要

New Brassica Ogura fertility restorer lines with a shortened Raphanus fragment are provided. The new lines lack the OPC2 marker and are capable of fully restoring fertility in Ogura cytoplasmic male sterile (cms) plants. The improved lines were developed using a new breeding method. The new breeding method can be used to shorten an exotic insertion comprising a gene of interest in any plant.

主权项

1. A Brassica plant comprising a fertility gene for Ogura cytoplasmic male sterility, wherein the fertility gene is on a Raphanus fragment introgressed from Raphanus sativa, and the Raphanus fragment lacks markers RMA01, RMA02, RMA03, RMA04, RMA05, RMA06, RMA07, RMA08, RMA09, RMA10, RMC24, OPC2, RMC25, RMC26, RMC27, RMC28, RMC29, RMC30, RMC31, RMC32 and RMC33; and wherein said Raphanus fragment comprises a molecular marker selected from the group consisting of RMB01, E35M62, RMB02, RMB03, RMB04, RMB05, RMB06, RMB07, RMB08, RMB09, RMB10, OPF10, RMB11, RMB12, RMC01, RMC02, RMC03, E38M60, RMC04, RMC05, RMC06, RMC07, RMC08, RMC17, RMC18, RMC19, RMC20, RMC21, RMC22 and RMC23.