Coated sensors and methods related thereto

摘要

The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.

主权项

1. A method for manufacturing a sensor for detecting an analyte, the method comprising:
forming a polymer matrix; adding a fluorescence source and a chromophore into the polymer matrix, wherein the chromophore binds to the moieties in the absence of the analyte; forming a sensor particle from the polymer matrix; and applying a surface coating to the sensor particle, wherein the surface coating creates a continuous coating of the sensor particle and the surface coating is penetrable by the analyte and at least partially covalently bound to the surface of the sensor particle.