Separation of pyrophosphate release and pyrophosphate detection

摘要

The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed herein are methods and systems which utilize improved pyrophosphate detection for nucleotide sequencing.

主权项

1. A method of delaying pyrophosphate detection comprising:
(a) incorporating a nucleotide or nucleotide analog into a polynucleotide, thereby generating pyrophosphate; (b) sequestering the pyrophosphate with a pyrophosphate sequestering agent; (c) releasing the pyrophosphate from the sequestering agent, thereby generating released pyrophosphate; and (d) detecting the released pyrophosphate.