Copolymer containing 3-hydroxyalkanoate unit and lactate unit, and its manufacturing method

摘要

The present invention relates to a copolymer comprising 3-hydroxyalkanoate monomer unit and lactate monomer unit, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer and 3-hydroxyalkanoate monomer, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used also for medical use.

主权项

1. A method for preparing a copolymer comprising a lactate monomer unit and a 3-hydroxyalkanoate monomer unit,
wherein the method comprises culturing a microorganism comprising a gene encoding an enzyme converting lactate into lactyl-CoA, a gene encoding an enzyme converting 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA, and a polyhydroxyalkanoate (PHA) synthase gene together, and wherein the PHA synthase gene encodes the amino acid sequence of SEQ ID NO: 8 having mutations of: a) S325T and Q481M; b) E130D and Q481K; c) S325T and Q481K; d) E130D and Q481M; e) E130D and Q481R; f) E130D, S325T and Q481M; g) E130D, S325T and Q481K; h) E130D, S477R and Q481K; i) E130D, S477R and Q481M; j) E130D, S477R and Q481R; k) E130D, S477H and Q481K; l) E130D, S477H and Q481M; m) E130D, S477H and Q481R; n) E130D, S477F and Q481K; o) E130D, S477F and Q481M; p) E130D, S477F and Q481R; q) E130D, S477Y and Q481K; r) E130D, S477Y and Q481M; s) E130D, S477Y and Q481R; t) E130D, S325T, S477R and Q481M; u) E130D, S325T, S477R and Q481K; v) E130D, S325T, S477F and Q481M; w) E130D, S325T, S477G and Q481M; or x) E130D, S325T, S477F and Q481K.