Method of making a formulation for deactivating nucleic acids

摘要

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

主权项

1. A method of making a formulation for deactivating nucleic acids, the method comprising the steps of:
(a) separately dissolving solid forms of a corrosion-inhibiting agent and a wetting agent, the corrosion-inhibiting agent being phosphate, borate or sodium bicarbonate, and the wetting agent being a detergent or a surfactant; (b) combining together the dissolved forms of the corrosion-inhibiting agent and the wetting agent to form a first mixture; (c) combining together the first mixture with a solubilizing agent to form a second mixture, the solubilizing agent being an organic solvent or an emulsifying agent; and (d) combining together the second mixture and a deactivating agent to form the formulation, the deactivating agent being sodium hypochlorite or dichloroisocyanurate, wherein the corrosion-inhibiting agent is present in the formulation at a concentration of from about 10 mM to about 750 mM; wherein the wetting agent is present in the formulation at a concentration of from about 0.005% to about 1% (w/v); wherein the solubilizing agent is present in the formulation at a concentration of from about 0.001% to about 20% (v/v); wherein the deactivating agent is (i) present in the formulation at a concentration of from about 0.06% to about 3% (w/v) if the deactivating agent is sodium hypochlorite; wherein the deactivating agent is present in the formulation at a concentration of from about 5 mM to about 400 mM if the deactivating agent is dichloroisocyanurate; and wherein each agent of the formulation remains substantially in solution at 22° C.