发明名称 |
OBSERVATION SUBSTRATE, WELL PLATE, SURFACE OBSERVATION DEVICE AND SURFACE OBSERVATION METHOD |
摘要 |
<p>PROBLEM TO BE SOLVED: To solve such problems that when an incident light microscope is used, fluorescence is excited not only on a cell surface but inside the cell to act as background light, which results in a low S/N, and that, whereas a confocal laser microscope and a total reflection microscope selectively excite fluorescence present on a specific surface of a cell and give a high resolution image with a high S/N, optical systems are complicated and devices are large and expensive.SOLUTION: An observation substrate for the observation of a sample surface is provided, which includes a body part that is transparent to light at a preliminarily determined wavelength. The body part has a varied surface area where the refractive index is varied at a predetermined pitch on one surface of the body part; the pitch is controlled to be within a ±10% range of an integral multiple of a length obtained by dividing a wavelength in a free space by the effective refractive index in the varied surface area.</p> |
申请公布号 |
JP2014119436(A) |
申请公布日期 |
2014.06.30 |
申请号 |
JP20120277329 |
申请日期 |
2012.12.19 |
申请人 |
INSTITUTE OF PHYSICAL &, CHEMICAL RESEARCH;KOBE UNIV |
发明人 |
AOKI HIROYOSHI;MIYOSHI HIROMI;AOKI KANNA;YAMAGATA YUTAKA |
分类号 |
G01N21/64 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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