METHOD OF PRODUCTION OF IMMUNOGENIC COMPOSITION BASED ON FUSION PROTEIN pESAT6-DBD AND DEXTRAN, RECOMBINANT PLASMID pESAT6-DBD, STRAIN OF Escherichia coli, CHIMERIC PROTEIN ESAT6-DBD AND THEIR APPLICATION

摘要

FIELD: biotechnology.SUBSTANCE: invention provides a recombinant plasmid pESAT6-DBD consisting of the artificial bacterial operon of chimeric protein comprising a promoter region of an early promoter of bacteriophage T5, gene of the chimeric protein consisting of a sequence of protein antigen ESAT6 of Mycobacterium tuberculosis, fused with the sequence of dextran-binding domain (DBD) of dextran-sucrase Leuconostoc citreum KM20 and transcription terminator; bacterial operon of beta-lactamase and bacterial initiation site of replication of ColEl type. The invention also comprises the strain of Escherichia coli - producer of the chimeric protein ESAT6-DBD, and the method of immobilisation, concentration and purification of the resulting protein on the dextran. In addition, the invention relates to the recombinant protein ESAT6-DBD and immunogenic compositions comprising it, aiming at the induction of immunity against tuberculosis infection.EFFECT: invention enables to obtain producing strain which provides high level of production of stable immunogenic proteins which can be prepared, immobilised and purified in a single step, and also to receive the effective immunogenic compositions against tuberculosis.5 cl, 2 dwg, 1 tbl, 5 ex