| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to a method of obtaining a preparation, stimulating antigen-independent differentiation of B-lymphocytes, from a raw material of an animal origin. The method of obtaining the preparation, stimulating antigen-independent differentiation of B-lymphocytes, from the raw material of the animal origin includes homogenisation of the preliminarily frozen at -40°C tissue of porcine endometrium with further extraction for 36 hours in 3% solution of acetic acid with a ratio 1 part of the raw material to 5 parts of an acetic acid solution and addition of zinc chloride in dose 1 g per 1 l of 3% acetic acid at pH 3.5-4.0, in which the obtained extract is filtered, centrifuged at 3000 rev/min for 20 min, a supernatant liquid is poured into the cooled acetone with a ratio 1:5, a sediment is formed for 12 hours at -4°C, precipitated with acetone, the target product is additionally purified by ultrafiltration with the limit of molecular retention 5kD, with further filtration and lyophilisation.EFFECT: extension of the assortment of highly efficient medications, stimulating antigen-independent differentiation of B-lymphocytes.5 tbl, 1 ex |
