| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to a method of identifying improved protein versions. The method includes testing a multitude of protein versions with single substitutions in the first test of the first property and in the second test of the second property. The parent protein property is given a value 1.0 in each test. The favourable first or second property possesses a value, exceeding 1.0, and extremely unfavourable first or second property has a value less than approximately 0.80. The said parent protein represents protease. The first property is stability, the second property is washing efficiency. Identification of substitution and introduction of the substitution into the protein to obtain the protein with multiple substitutions are carried out. The substitution does not interact in a three-dimensional structure of the said parent protein, and one of the substitutions contains a change of the total charge, equal to 0, -1 or -2 with respect to the parent protein, and, at least, one of the substitutions contains the change of the total charge, equal to +1 or +2 with respect to the parent protein. The protein version with multiple changes in the first and second tests is tested and the said protein version is identified.EFFECT: invention makes it possible to obtain the protein version with improved stability and washing efficiency.14 cl, 6 dwg, 6 tbl, 7 ex |
