发明名称 |
CHROMATOGRAPHIC METHOD FOR ISOLATING AND PURIFYING HIGH-PURITY RECOMBINED HUMAN SERUM ALBUMIN |
摘要 |
Disclosed is a chromatographic method for isolating and purifying high-purity recombined human serum albumin, comprising: conducting cation-exchange chromatography on recombined human serum albumin, and adding alcohol to a buffer solution to remove endotoxin, so as to obtain a primary product I; conducting hydrophobic composite filler/anion-exchange chromatography on the primary product I to obtain an intermediate product II; conducting hydrophobic chromatography on the intermediate product II to obtain the target product, the purified high-purity recombined human serum albumin. The purity of the recombined human serum albumin obtained by isolating and purifying with the chromatographic method of the present invention is greater than 99.9999%, and the content of the endotoxin meets the criteria stipulated by Chinese pharmacopoeia. |
申请公布号 |
WO2014094406(A1) |
申请公布日期 |
2014.06.26 |
申请号 |
WO2013CN75405 |
申请日期 |
2013.05.09 |
申请人 |
HEALTHGEN BIOTECHNOLOGY CO., LTD. |
发明人 |
YANG, DAICHANG;SHI, BO;SHI, QIANNI;OU, JIQUAN;LIU, JINGRU |
分类号 |
C07K1/18;C07K14/765 |
主分类号 |
C07K1/18 |
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