POST-SYNTHETIC ORTHOGONAL AMIDATION PLUS METAL CATALYZED AZIDE-ALKYNE CYCLOADDITION CLICK CHEMISTRY ON siRNA
摘要
This invention relates to the post-synthetic chemical modifications of RNA at the 2' -position on the ribose rings via orthogonal chemistry involving amidation reactions plus metal catalyzed alkyne-azide cycloaddition (click) reactions. RNA interference (RNAi) is an evolutionarily conserved cellular mechanism of post-transcriptional gene silencing found in fungi, plants and animals that uses small RNA molecules to inhibit gene expression in a sequence-specific manner. The RNAi machinery can be harnessed to destruct any mRNA of a known sequence.
申请公布号
WO2014100069(A1)
申请公布日期
2014.06.26
申请号
WO2013US75914
申请日期
2013.12.18
申请人
MERCK SHARP & DOHME CORP.;ZEWGE, DANIEL;COPELAND, GREGORY, T.;LI, ZHEN;ARMSTRONG, JOSEPH, D., III
发明人
ZEWGE, DANIEL;COPELAND, GREGORY, T.;LI, ZHEN;ARMSTRONG, JOSEPH, D., III