YIELD TRAITS FOR MAIZE

摘要

Methods for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm are provided. In some embodiments, the methods include providing a Zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into Zea mays germplasm that lacks the allele. Also provided are methods for identifying Zea mays plants that contain at least one allele associated with improved yield, improved maize plants, elite Zea mays plants, biomass produced from improved Zea mays plants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying a Zea mays nucleic acid to generate a Zea mays marker amplicon.

主权项

1. A method for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm, the method comprising:
(a) selecting a Zea mays plant that comprises an allele of interest of a locus associated with a yield trait, identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers selected from among:
(1) primer pair 1 represented by a primer comprising SEQ ID NO: 52 and a primer comprising SEQ ID NO: 53;(2) primer pair 2 represented by a primer comprising SEQ ID NO: 57 and a primer comprising SEQ ID NO: 58;(3) primer pair 3 represented by a primer comprising SEQ ID NO: 62 and a primer comprising SEQ ID NO: 63;(4) primer pair 4 represented by a primer comprising SEQ ID NO: 67 and a primer comprising SEQ ID NO: 68;(5) primer pair 5 represented by a primer comprising SEQ ID NO: 72 and a primer comprising SEQ ID NO: 73;(6) primer pair 6 represented by a primer comprising SEQ ID NO: 77 and a primer comprising SEQ ID NO: 78;(7) primer pair 7 represented by a primer comprising SEQ ID NO: 82 and a primer comprising SEQ ID NO: 83;(8) primer pair 8 represented by a primer comprising SEQ ID NO: 87 and a primer comprising SEQ ID NO: 88;(9) primer pair 9 represented by a primer comprising SEQ ID NO: 92 and a primer comprising SEQ ID NO: 93;(10) primer pair 10 represented by a primer comprising SEQ ID NO: 97 and a primer comprising SEQ ID NO: 98;(11) primer pair 11 represented by a primer comprising SEQ ID NO: 102 and a primer comprising SEQ ID NO: 103;(12) primer pair 12 represented by a primer comprising SEQ ID NO: 107 and a primer comprising SEQ ID NO: 108;(13) primer pair 13 represented by a primer comprising SEQ ID NO: 112 and a primer comprising SEQ ID NO: 113;(14) primer pair 14 represented by a primer comprising SEQ ID NO: 117 and a primer comprising SEQ ID NO: 118;(15) primer pair 15 represented by a primer comprising SEQ ID NO: 122 and a primer comprising SEQ ID NO: 123;(16) primer pair 16 represented by a primer comprising SEQ ID NO: 127 and a primer comprising SEQ ID NO: 128;(17) primer pair 17 represented by a primer comprising SEQ ID NO: 132 and a primer comprising SEQ ID NO: 133;(18) primer pair 18 represented by a primer comprising SEQ ID NO: 137 and a primer comprising SEQ ID NO: 138;(19) primer pair 19 represented by a primer comprising SEQ ID NO: 142 and a primer comprising SEQ ID NO: 143;(20) primer pair 20 represented by a primer comprising SEQ ID NO: 147 and a primer comprising SEQ ID NO: 148;(21) primer pair 21 represented by a primer comprising SEQ ID NO: 152 and a primer comprising SEQ ID NO: 153;(22) primer pair 22 represented by a primer comprising SEQ ID NO: 157 and a primer comprising SEQ ID NO: 158;(23) primer pair 23 represented by a primer comprising SEQ ID NO: 162 and a primer comprising SEQ ID NO: 163;(24) primer pair 24 represented by a primer comprising SEQ ID NO: 167 and a primer comprising SEQ ID NO: 168;(25) primer pair 25 represented by a primer comprising SEQ ID NO: 172 and a primer comprising SEQ ID NO: 173;(26) primer pair 26 represented by a primer comprising SEQ ID NO: 177 and a primer comprising SEQ ID NO: 178;(27) primer pair 27 represented by a primer comprising SEQ ID NO: 182 and a primer comprising SEQ ID NO: 183;(28) primer pair 28 represented by a primer comprising SEQ ID NO: 187 and a primer comprising SEQ ID NO: 188;(29) primer pair 29 represented by a primer comprising SEQ ID NO: 192 and a primer comprising SEQ ID NO: 193;(30) primer pair 30 represented by a primer comprising SEQ ID NO: 197 and a primer comprising SEQ ID NO: 198;(31) primer pair 31 represented by a primer comprising SEQ ID NO: 202 and a primer comprising SEQ ID NO: 203;(32) primer pair 32 represented by a primer comprising SEQ ID NO: 207 and a primer comprising SEQ ID NO: 208;(33) primer pair 33 represented by a primer comprising SEQ ID NO: 212 and a primer comprising SEQ ID NO: 213;(34) primer pair 34 represented by a primer comprising SEQ ID NO: 217 and a primer comprising SEQ ID NO: 218;(35) primer pair 35 represented by a primer comprising SEQ ID NO: 222 and a primer comprising SEQ ID NO: 223;(36) primer pair 36 represented by a primer comprising SEQ ID NO: 227 and a primer comprising SEQ ID NO: 228;(37) primer pair 37 represented by a primer comprising SEQ ID NO: 232 and a primer comprising SEQ ID NO: 233;(38) primer pair 38 represented by a primer comprising SEQ ID NO: 237 and a primer comprising SEQ ID NO: 238;(39) primer pair 39 represented by a primer comprising SEQ ID NO: 242 and a primer comprising SEQ ID NO: 243;(40) primer pair 40 represented by a primer comprising SEQ ID NO: 247 and a primer comprising SEQ ID NO: 248;(41) primer pair 41 represented by a primer comprising SEQ ID NO: 252 and a primer comprising SEQ ID NO: 253;(42) primer pair 42 represented by a primer comprising SEQ ID NO: 257 and a primer comprising SEQ ID NO: 258;(43) primer pair 43 represented by a primer comprising SEQ ID NO: 262 and a primer comprising SEQ ID NO: 263;(44) primer pair 44 represented by a primer comprising SEQ ID NO: 267 and a primer comprising SEQ ID NO: 268;(45) primer pair 45 represented by a primer comprising SEQ ID NO: 272 and a primer comprising SEQ ID NO: 273;(46) primer pair 46 represented by a primer comprising SEQ ID NO: 277 and a primer comprising SEQ ID NO: 278;(47) primer pair 47 represented by a primer comprising SEQ ID NO: 282 and a primer comprising SEQ ID NO: 283;(48) primer pair 48 represented by a primer comprising SEQ ID NO: 287 and a primer comprising SEQ ID NO: 288;(49) primer pair 49 represented by a primer comprising SEQ ID NO: 292 and a primer comprising SEQ ID NO: 293;(50) primer pair 50 represented by a primer comprising SEQ ID NO: 297 and a primer comprising SEQ ID NO: 298; and(51) primer pair 51 represented by a primer comprising SEQ ID NO: 302 and a primer comprising SEQ ID NO: 303; and (b) introgressing the allele of interest into Zea mays germplasm that lacks the allele.