METHODS FOR THE SELECTION OF BINDING PROTEINS

摘要

This application provides an improved screening method for the selection of target-binding proteins having desirable biophysical properties. The method combines mRNA display and yeast surface display in a way that takes advantage of the desirable attributes of both processes.

主权项

1. A method for selecting at least one target-binding protein with favorable biophysical properties, comprising
a first phase comprising selecting a first population of protein variants binding a target protein (“target-binding protein variants”) from a library comprising at least about 1012 protein variants; and a second phase comprising selecting a second population of target-binding protein variants from at least a portion of the first population of target-binding protein variants using yeast surface display, wherein the second population of target-binding protein variants comprises at least one target-binding protein with favorable biophysical properties.