主权项 |
1. A method of determining the likelihood of a test individual having a critical and/or life threatening response to a suspected illness, said method comprising:
(i) detecting and quantifying a level of each of two or more protein biomarkers in a sample from the test individual, wherein the test individual has not been diagnosed or differentially diagnosed as having the suspected illness, wherein said protein biomarkers are: complement fragment C5a (C5a), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), 10 kDa interferon gamma-induced protein (IP-10), soluble tyrosine kinase with immunoglobulin-like loop and epidermal growth factor domain-2 (sTie-2), soluble intercellular adhesion molecule-1 (sICAM-1), vascular endothelial growth factor A (VEGF), soluble vascular endothelial growth factor receptor 1 (sFlt-1), Chitinase-3-like protein 1 (CHI3L1), soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), C-reactive protein (CRP), procalcitonin (PCT), Angiopoietin-like protein 3 (Ang-like 3), complement factor D (Factor D), or interleukin 18 binding protein (IL18bpa), endoglin (End), p-selectin (p-SEL), von Endothelial soluble Tie-2 Receptor (Tie-2) and Willibrand Factor (vWF) as set out in Table 1; (ii) comparing said quantified levels of said protein biomarkers to control levels of said protein biomarkers from a control population; and (iii) determining the differential levels for each biomarker in the comparison of step (ii) so as to make a determination as to whether said test individual is at an increased risk of having the critical and/or life threatening response. |