METHOD FOR PRODUCING HETEROGENOUS EMBRYONIC CHIMERIC ANIMAL USING STEM CELL

摘要

PROBLEM TO BE SOLVED: To provide a technique capable of quickly and simply creating an embryonic chimeric animal among heterogeneous animals using a stem cell.SOLUTION: A method for preparing a chimeric animal comprises the steps of: (A) injecting a stem cell into a blastocyst cavity in a blastocyst stage of an animal heterologous to that of the stem cell, or mixing the stem cell with a divided fertilized egg of the animal heterologous to that of the stem cell; and (B) growing a cell mass including the stem cell prepared in the step (A) into a chimeric animal between a species of the stem cell and a species of the heterologous animal. The stem cell is any one of an embryonic stem (ES) cell and an induced pluripotent stem (iPS) cell.