| 摘要 |
The present invention discloses a stem cell culture medium and its applications as well as a stem cell culture method. The said stem cell culture medium contains no serum. The said stem cell culture medium contains amino acids, vitamins, salts, lipids, cytokines and protein polypeptides. The said stem cell culture medium is suitable for rapid culture of stem cells derived from human and mammalian tissues, including but not limited to, adipose mesenchymal stem cells, bone marrow mesenchymal stem cells and umbilical cord blood stem cells. The said culture medium can increase the proliferation speed of the stem cells 3-5 times, without any affects on their differentiation potentials. Comparing the said stem cell culture medium to a routine culture medium, the said culture medium is not only able to proliferate stem cells derived from different sources more rapidly and achieve more proliferation generations, but also keep their differentiation potentials well. |
| 主权项 |
1. A stem cell culture medium, wherein, the said culture medium contains no serum, and the said stem cells culture medium contains amino acids, vitamins, salts, lipids, cytokines and protein polypeptides;
the said amino acids include Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine, Glutamine; the said cytokines and protein polypeptides include fibroblast growth factor 1, fibroblast growth factor 2, epidermal growth factor, platelet-derived growth factor, insulin, insulin-like growth factor 1, vascular endothelial cell growth factor, placenta growth factor, leukocyte inhibitor factor, stem cell factor, transferrin and human serum albumin; the said vitamins include, biotin, choline chloride, D-pantothenic acid sodium, folic acid, inositol, niacinamide, riboflavin, pyridoxine hydrochloride, thiamine hydrochloride, coenzyme Q10, vitamin B12, putrescine dihydrochloride, vitamin C and vitamin E; the said lipids include dexamethasone, oleic acid, cholesterol, monoethanolamine, linoleic acid, lipoic acid and lipid mixtures; the said salts include sodium bicarbonate, calcium chloride, potassium chloride, magnesium chloride, magnesium sulfate, sodium chloride, monohydrate sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium pyruvate;the stem cell culture medium include antioxidant, D-glucose, taurine, sodium heparin. |
