DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS
摘要
Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.
申请公布号
WO2014100061(A1)
申请公布日期
2014.06.26
申请号
WO2013US75895
申请日期
2013.12.18
申请人
E. I. DU PONT DE NEMOURS AND COMPANY
发明人
ZHU, QUINN, QUN;HONG, SEUNG-PYO;XIE, DONGMING;XUE, ZHIXIONG;YOON, HYERYOUNG;DAUNER, MICHAEL