| 摘要 |
PROBLEM TO BE SOLVED: To provide a newly isolated 4-keto-arabonic acid synthetic enzyme that catalyzes both reactions of decarboxylation and oxidation in formation of 4-keto-D-arabonic acid from 2,5-diketogluconic acid.SOLUTION: Provided is a 4-keto-arabonic acid synthetic enzyme having the following physicochemical properties: (a) optimum pH: 4.5-7.0; (b) optimum temperature: 0°C-60°C; (c) molecular size: 92,200±3,500 dalton (two subunits of which molecular size is 46,100±1,500 dalton); and (e) coenzyme: thiamine pyrophosphate, pyrroloquinoline quinone. |
