IMPROVED SPLIT SEED SOYBEAN TRANSFORMATION FOR EFFICIENT AND HIGH-THROUGHPUT TRANSGENIC EVENT PRODUCTION

摘要

A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, retaining a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.