METHODS AND DEVICES SUITABLE FOR IMAGING BLOOD-CONTAINING TISSUE

摘要

Disclosed are methods and devices useful for imaging blood-containing tissue, for example for angiography, especially retinal angiography, whereby an image is generated by dividing pixels of an image acquired at some wavelength range by corresponding pixels of an image acquired at a different wavelength range. In some embodiments, a first wavelength range includes predominantly light having wavelengths between about 400 nm and about 620 nm and a second wavelength range includes predominantly light having wavelengths between about 620 nm and about 800 nm.

主权项

1. A method of generating an image of the surface of biological tissue, comprising:
a) illuminating an area of interest of the surface with light comprising wavelengths within a first wavelength range, said first wavelength range including predominantly light having wavelengths of between about 400 nm and about 620 nm; b) acquiring a first pixelated image of light reflected from said area of interest of the surface at said first wavelength range of light; c) illuminating said area of interest of the surface with light comprising wavelengths within a second wavelength range, said second wavelength range including predominantly light having wavelengths of between about 620 nm and about 800 nm; d) acquiring a second pixelated image of light reflected from said area of interest of the surface at said second wavelength range of light; and e) generating a monochromatic third pixelated image from said first image and said second image by:
for each desired location i of said area of interest, identifying a corresponding pixel P1(i) in said first image and a corresponding pixel P2(i) in said second image; andcalculating a pixel P3(i) in said third image corresponding to said location i, by dividing one of a mathematical formula of P1(i) and a mathematical formula of P2(i) by the other.