METHODS AND COMPOSITIONS FOR IMPROVED PRODUCTION OF FATTY ACIDS AND DERIVATIVES THEREOF

摘要

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.

主权项

1. A method of producing a fatty acid or derivative thereof, comprising:
(a) providing a host cell which is genetically engineered to have an altered level of expression of a FadR polypeptide as compared to the level of expression of the FadR polypeptide in a corresponding wild-type host cell, (b) culturing the engineered host cell in a culture medium under conditions permissive for the production of a fatty acid or derivative thereof, and (c) isolating the fatty acid or derivative thereof from the engineered host cell, wherein one or more of the titer, yield, or productivity of the fatty acid or derivative thereof produced by the engineered host cell is increased relative to that of the corresponding wild-type host cell.