发明名称 |
Methods for producing retinal tissue and retina-related cell |
摘要 |
The present invention provides: [1] a method for producing a retinal tissue, characterized by comprising steps (1) to (3), i.e., (1) a first step of subjecting a pluripotent stem cell to floating culture in a serum-free culture medium containing a Wnt signaling pathway inhibitor to form an aggregate of the pluripotent stem cell, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free culture medium containing a basement membrane preparation, and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing culture medium; [2] a method for producing an optic-cup-like structure, characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum-free culture medium or a serum-containing culture medium each containing a substance capable of acting on the Sonic hedgehog signaling way and a substance capable of acting on the Wnt signaling pathway; [3] a method for producing a retinal pigment epithelial cell, characterized by comprising a step of subjecting the aggregate containing a retinal tissue which has been cultured in the third step mentioned above to floating culture in a serum-free culture medium or a serum-containing culture medium each containing a substance capable of acting on the Wnt signaling pathway (wherein the serum-free culture medium and the serum-containing culture medium does not contain any substance capable of acting on the Sonic hedgehog signaling pathway); and [4] a method for producing a retinal-layer-specific nerve cell, characterized by comprising bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a Notch signaling pathway inhibitor. |
申请公布号 |
AU2012341417(A1) |
申请公布日期 |
2014.06.26 |
申请号 |
AU20120341417 |
申请日期 |
2012.11.22 |
申请人 |
RIKEN;SUMITOMO CHEMICAL COMPANY, LIMITED |
发明人 |
NAKANO, TOKUSHIGE;ANDO, SATOSHI;SASAI, YOSHIKI;EIRAKU, MOTOTSUGU |
分类号 |
C12N5/071;A61L27/00;C12N5/0789;C12N5/079;C12Q1/02;G01N33/15;G01N33/50 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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