| 摘要 |
Determining a degree of immunosuppression in an individual treated with an immunosuppressive agent, comprises: (a) determining virus-specific cluster of differentiation 4 (CD4) T-cells after in vitro stimulation with the virus-specific antigens in a blood sample of the individual; and (b) determining a level of immunosuppression based on the level or the number of virus-specific CD4 T-cells determined in the step (a). Independent claims are included for: (1) a method-I for the stratification of immunosuppression and the control of the therapeutic regimen in the individual treated with the immunosuppressive agent, comprising the steps (a) and (b) as per above se, and (c1) determining the further dosage of other immunosuppressive therapy on the basis of the immunosuppression degree according to the step (b); (2) a method-II for monitoring the immunosuppression in the individual treated with the immunosuppressive agent, comprising the step (a) as per above se, (b1) determining the virus-specific CD4 T-cells after in vitro stimulation with the virus-specific antigens in a second blood sample of an individual obtained from the first blood sample, and (c2) comparing the level or the amount of virus-specific CD4 T-cells as determined in the step (a) with the level or the amount determined in the step (b) or with a reference value, where an increase in the level or the number of virus-specific CD4 T-cells is indicative for a reduction of immunosuppression, and a decrease in the level or the amount of virus-specific CD4 T-cells is indicative for a reinforced immunosuppression; and (3) use of a kit or assays to determine the degree of immunosuppression in an individual treated with the immunosuppressive agent, to control the immunosuppression and the therapeutic regimen in an individual treated with an immunosuppressive agent, to determine the change in the immunosuppression of the individual, and/or to monitor the immunosuppression in the individual, comprising an agent for the determination of virus-specific CD4 T-cells after in vitro stimulation with the virus-specific antigens, and instructions to perform the assay or kit. |
