Methods for detecting vitamin C by mass spectrometry

摘要

Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.

主权项

1. A method for determining an amount of vitamin C in a test sample, the method comprising:
(i) ionizing vitamin C from the test sample and an internal standard to generate at least one vitamin C ion and at least one internal standard ion detectable by mass spectrometry, wherein the at least one vitamin C ion comprises a precursor ion with a mass/charge ratio of 175.05±0.5; and (ii) comparing amounts of the ions generated in (i) for respectively the at least one vitamin C ion and the at least one internal standard ion to determine the amount of vitamin C in a test sample from the comparison.