| 发明名称 | Method of cell culture | ||
| 摘要 | This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated. | ||
| 申请公布号 | US8759100(B2) | 申请公布日期 | 2014.06.24 |
| 申请号 | US201013990642 | 申请日期 | 2010.12.06 |
| 申请人 | Dai Nippon Printing Co., Ltd. | 发明人 | Yokoyama Naoki;Akai Tomonori |
| 分类号 | C12N5/00 | 主分类号 | C12N5/00 |
| 代理机构 | Lucas & Mercanti, LLP | 代理人 | Lucas & Mercanti, LLP |
| 主权项 | 1. A method of cell culture comprising: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated. | ||
| 地址 | Tokyo JP | ||