| 摘要 |
PURPOSE: An antioxidative composition for culturing mesenchymal stromal cells is provided to effectively improve proliferation or differentiation of stromal cells caused by oxidative stress. CONSTITUTION: An antioxidative composition for culturing mesenchymal stromal cells contains necrostatin as an active ingredient. The concentration of nectrostatin is 1-50 uM. The composition increases the expression of cytokines or nerve growth factors. The cytokines or nerve growth factors are Oct4, placental growth factor (PGF), fibroblast growth factor 1 (FGF1), insulin-like growth factor 1 (IGF-1), transforming growth factor alpha inositol hexaphosphate TNFalphaIP6 (TGFalphaIP6), TGFbeta1, interleukin 4 (IL4), or IL10. A method for in vitro expansion of mesenchymal stromal cells comprises the step of culturing the mesenchymal stromal cells in a medium containing necrostatin. |
