主权项 |
1. A dye having the structure: wherein
at least one of R8 and R21 or R7 and R22 are taken together to form a five-membered or six-membered saturated or unsaturated heteroalkyl ring;at least one of R1, R2, R3, R6, R7, R8R9, R21 or R22 is Q, wherein Q is selected from a sulfoxide (SOR13), a sulfone (SO2CR13R14R15), a sulfonamide (SO2NR13R14), a phosphonate (PO3═), a phosphonate monoester (PO2−ER13) a phosphonate dioster (POER13ER14), a thiophosphate (PSO3═), a thiophosphate monoester (PSO2−ER13) a thiophosphate diester (PSOER13ER14), a thiophosphonate (PSO2═), a thiophosphonate monoester (PSO−ER13) a thiophosphonate diester (PSER13ER14), a phosphonamide (PONR13R14NR19R20), a thioanalogue of a phosphonamide, (PSNR13R14NR19R20), a phosphoramide (PONR13R14NR15NR19R20), a thioanalogue of a phosphoramide (PSNR13R14NR15NR19R20), a phosphoramidite (PO2R19NR13R14), a thioanalogue of a phosphoramidite (POSR19R13R14), wherein E is O or S;wherein Q is attached directly, or indirectly through a saturated or unsaturated, linear or branched, substituted or unsubstituted carbon linker arm, which may further comprise one or more heteroatoms selected from sulfur, oxygen and nitrogen, and wherein when Q is a sulfonamide, it does not comprise a terminal reactive group or a linker joining the dye to a target molecule;R13, R14, R15, R19 and R20 are independently selected from hydrogen, halogen, an amino group, a saturated or unsaturated, linear or branched, substituted or unsubstituted alkyl group, or when taken together R13 and R14 form a five-membered or six-membered saturated or unsaturated heteroalkyl ring;wherein the remaining R1, R2, R3, R5, R6, R7, R8, R9, R21 and R22 are independently selected from hydrogen, Z, a saturated or unsaturated, linear or branched, substituted or unsubstituted alkyl group, a saturated or unsaturated, linear or branched, substituted or unsubstituted alkyl group alkoxy group, or when taken together, R2 and R3, R6 and R7, and R8 and R9 form a five-membered or six-membered ring;Z is selected from a carboxyl group (CO2−), a carbonate ester (COER13), a sulfonate (SO3−), a sulfonate ester (SO2ER13), a sulfoxide (SOR13), a sulfone (SO2CR13R14R15), a sulfonamide (SO2NR13R14), a phosphate (PO4═), a phosphate monoester (PO2−ER13), a phosphate diester (PO2ER13ER14), a phosphonate (PO3═) a phosphonate monoester (PSO2−ER13) a phosphonate diester (POER13ER14), a thiophosphate (PSO3═), a thiophosphate monoester (PSO2−ER13) a thiophosphate diester (PSOER13ER14), a thiophosphonate (PSO2═), a thiophosphonate monoester (PSO−ER13) a thiophosphonate diester (PSER13ER14), a phosphonamide (PONR13R14NR19R20), a thioanalogue of a phosphonamide (PSNR13R14NR19R20), a phosphoramide (PONR13R14NR15NR19R20), a thioanalogue of a phosphoramide (PSNR13R14NR15NR19R20), a phosphoramidite (PO2R19NR13R14) or a thioanalogue of a phosphoramidite (POSR19NR13R14), and E is O or S;wherein Z is attached directly or indirectly through a saturated or unsaturated, linear or branched, substituted or unsubstituted carbon linker arm, which may further comprise one or more heteroatoms selected from sulfur, oxygen and nitrogen;and wherein any of R1, R2, R3, R5, R6, R7, R8, R9, R21 or R22 may further comprise a heteroatom containing side chain wherein said side chain is joined to the R group by a linkage selected from an ether linkage (—OR25), a thioether linkage (—SR25), and an amine linkage (—NR25R26 or —N+R25R26R27), andwherein R25, R26 and R27 are each independently selected from hydrogen, Z, a saturated or unsaturated, linear or branched, substituted or unsubstituted alkyl group, a saturated or unsaturated, linear or branched, substituted or unsubstituted alkoxy group, or when taken together, R25 and R26, or R26 and R27 form a saturated or unsaturated five-membered or six-membered alkyl or heteroalkyl ring, and wherein any of R25, R26 or R27 may further comprise a heteroatom containing side chain.
|