Two-photon absorbing water soluble fluorescent probe as a near-neutral pH indicator

摘要

Disclosed are fluorescent compounds useful as intracellular pH probes. In particular, the invention teaches a two-photon absorbing, water soluble, fluorescent compound, a fluorene derivative, which is effective as a near-neutral pH indicator and particularly as an intracellular probe. A method for chemical synthesis of the claimed compounds is provided.

主权项

1. A method of sensing pH inside a cell, the method comprising introducing a fluorescent compound having the following structure into the cell, irradiating the cell with a near-infrared wavelength, detecting fluorescence from the compound, and associating the fluorescence with a pH, wherein R1=ethyl and R2=C2H4COOH.