发明名称 |
Process for producing prenyl alcohols |
摘要 |
A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture. |
申请公布号 |
US8759046(B2) |
申请公布日期 |
2014.06.24 |
申请号 |
US200812060434 |
申请日期 |
2008.04.01 |
申请人 |
Toyota Jidosha Kabushiki Kaisha |
发明人 |
Ohto Chikara;Obata Shusei |
分类号 |
C12P7/02;C12N1/00;C12N15/00;C07H21/04 |
主分类号 |
C12P7/02 |
代理机构 |
Kenyon & Kenyon LLP |
代理人 |
Kenyon & Kenyon LLP |
主权项 |
1. A method of producing a prenyl alcohol(s) selected from the group consisting of farnesol, nerolidol and geranylgeraniol, comprising the steps of:
culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced to produce a fusion protein having both farnesyl diphosphate synthase activity and geranylgeranyl diphosphate synthase activity; wherein the mutant cell is derived from a mutant strain of Saccharomyces cerevisiae, and wherein the mutant strain is prepared by replacing a transcription promoter of a squalene synthase gene with a transcription repression type promoter; and recovering the prenyl alcohol(s) selected from the group consisting of farnesol, nerolidol and geranylgeraniol from the resultant culture; wherein the farnesol diphosphate (FPP) synthase gene encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:2 or 4 or a polypeptide having FPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:2 or 4 by deletion, substitution, or addition of from one to ten amino acids; and wherein the geranylgeranyl diphosphate (GGPP) synthase gene encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:6 or a polypeptide having GGPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:6 by deletion, substitution, or addition of from one to ten amino acids.
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地址 |
Toyota-Shi JP |