Thrombin activator compositions and methods of making and using the same

摘要

Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.

主权项

1. A recombinant metalloprotease pre-pro-activator comprising, from an amino-terminal position to a carboxyl-terminal position, a pre-pro leader that shares at least 60% sequence identity with the pre-pro leader from Echis carinatus ecarin wild-type metalloprotease pre-pro-activator; a thrombin cleavage site consisting of a glycine, a proline, and an arginine; and a mature activator that shares at least 60% sequence identity with the mature activator from the Echis carinatus ecarin wild-type metalloprotease pre-pro-activator.