Methods for generating or increasing revenues from crops

摘要

The present invention provides methods of doing business and providing services. For example, methods of increasing the revenue of crops are provided. To this end, the method includes the use of a nucleic acid sequences of plant centromeres. This will permit construction of stably inherited recombinant DNA constructs and mini chromosomes which can serve as vectors for the construction of transgenic plant and animal cells.

主权项

1. A method for generating revenue based on agricultural products comprising the steps of:
developing a new crop comprising a mini-chromosome including a sequence of a crop plant centromere, wherein the crop plant centromere:
(i) comprises n copies of a repeated nucleotide sequence from centromeric genomic DNA of the plant, wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs, and wherein n is at least 5; and(ii) confers an ability to a nucleic acid comprising the plant centromere to segregate to daughter cells through cell division; and receiving a fee for the new crop.