| 摘要 |
FIELD: medicine.SUBSTANCE: course of radiation and/or chemotherapy is preceded by laparoscopic sampling of ovarian tissue from a cortical layer, and fragmentation by 2-5 mm. One portion of the samples is taken to histological studies, while the other one is placed in cryotubes for two-staged preservation. A cryoprotector is presented by 10-14% dimethyl sulphoxide at the first stage, and 20% 2.5 M Saccharose and 29-32% dimethyl sulphoxide at the second stage. In the process of autografting, the ovarian tissue samples are de-frozen with using Kitazata medium and assessed by a histological examination. Immediately before the transplantation, a laparoscopic biopsy of the preserved ovaries is followed by the histological examination. If the examination result is satisfactory, a bed is formed in the cortical layer of each ovary to place therein 5 to 10 fragmented de-frozen samples. Bed edges are closed, processed with biologically neutral glue and clamped for the purpose of further visual inspection and follicle growth estimate of the transplant tissue with using a visual diagnostic technique. Hormone concentrations are monitored once a month until the hormone concentration actual for the moment of ovarian tissue sampling for preservation is reached.EFFECT: method enables preserving or recovering the reproductive function in females suffering from oncological diseases by transplanting the de-cryopreserved ovarian tissue into the preserved ovaries that provides the adequate vascularisation and tissue survival rate.4 cl, 3 tbl, 5 dwg, 3 ex |
