发明名称 HAV detection
摘要 Methods for detecting HAV in a biological sample are provided, comprising amplifying a target nucleic acid comprising the sequence of HAV in a reaction mixture. The reaction mixture comprises a biological sample which may contain the target nucleic acid and set of oligonucleotides. The invention also provides kits for the detection of HAV.
申请公布号 US2014170638(A1) 申请公布日期 2014.06.19
申请号 US201314088007 申请日期 2013.11.22
申请人 ROCHE MOLECULAR SYSTEMS, INC. 发明人 Leying Hermann;Lu Ning;Newton Nick;Wolfelschneider Andreas;Young Karen;Zimmermann Dirk
分类号 C12Q1/70 主分类号 C12Q1/70
代理机构 代理人
主权项 1. A method of detecting HAV in a biological sample, comprising a) amplifying a target nucleic acid comprising the nucleic acid sequence of HAV in a reaction mixture, the reaction mixture comprising a biological sample which may contain the target nucleic acid, and at least a first and a second primer, wherein the nucleic acid sequence of the first primer is selected from SEQ ID NOS: 5-8 and the nucleic acid sequence of the second primer is selected from SEQ ID NOS: 10-11; wherein the amplifying generates an amplified target nucleic acid, and b) detecting the amplified target nucleic acid.
地址 PLEASANTON CA US